Description Versatile, comprehensive, and clearly written, this competitively priced laboratory manual can be used with any undergraduate microbiology text–and now features brief clinical applications for each experiment, MasteringMicrobiology ® quizzes that correspond to each experiment, and a new experiment on hand washing. Microbiology: A Laboratory Manual is known for its thorough coverage, descriptive and straightforward procedures, and minimal equipment requirements. A broad range of experiments helps to convey basic principles and techniques. Each experiment includes an overview, an in-depth discussion of the principle involved, easy-to-follow procedures, and lab reports with review and critical thinking questions. Ample introductory material and laboratory safety instructions are provided. Comprehensive coverage of the core microbiology topics includes experiments in the areas of genetics, immunology, and biotechnology. A wide range of experiments progressing from simple to complex enable instructors to tailor their laboratory classes to the topics they wish to cover.
Experiments use the most common and affordable laboratory materials, designed to accommodate any lab. Over 90 photographs in full color and numerous illustrations appear directly alongside the experiments, helping students visualize techniques and expected results. Spiral binding makes student-use easier and minimizes space on a lab bench. A detailed introductory section on basic lab techniques and safety thoroughly prepares students for lab work during the semester. “Caution” icons alert users to experiments that pose a potential risk. Six appendices cover the topics of Scientific Notification, Methods for Preparation of Dilutions, Microbiological Media, Biochemical Test Reagents, Staining Reagents, and Experimental Microorganisms. A Guide to Serial Dilutions is printed on the inside back cover for students’ quick reference in completing exercises.
Art demonstrating lab procedures appears consistently in a special box design that distinguishes it from other art, and catches the student’s eye. A bold and modern four-color design adds distinction to each individual element, and allows for easier navigation within each experiment.
Clinical Applications have been integrated into each lab. This feature helps students understand why they’re learning each technique and make the connection between lab, lecture, and their future careers as nurses or in other allied health fields. Hand washing experiment introduces the importance of appropriate hand washing and helps introduce students to microbiology in a fun, easy way.
Ten MasteringMicrobiology ® quiz questions have been added for each experiment. Each question is tied to one of the Learning Objectives in the lab. Additional photos of fungi and protozoa have been added to the manual. The slide method has been added to the Catalase Test experiment (Experiment 30). The filter paper method has been added to the Oxidase Test experiment (Experiment 31).
NEW to MasteringMicrobiology:. MicroLab Tutors help instructors and students get the most out of lab time.
Using a combination of videos, animations, assessment and visual feedback, these tutorials are designed to make sure that students come better prepared for the lab by introducing and assessing student understanding of lab concepts and techniques outside of formal lecture and lab time. MicroLab Tutors are assessable and assignable in MasteringMicrobiology. Lab Technique Videos give students an opportunity to see techniques performed and quiz themselves on lab procedures both before and after lab time. Students arrive more prepared and confident for lab time, freeing up valuable lab and lecture time. These videos also provide students valuable study opportunities before their lab practicals. Table of Contents Part 1: Basic Laboratory Techniques for Isolation, Cultivation, and Cultural Characterization of Microorganisms 1. Effectiveness of Hand Washing 2. Culture Transfer Techniques 3.
Techniques for Isolation of Pure Cultures 4. Cultural Characteristics of Microorganisms Part 2: Microscopy 5. Microscopic Examination of Stained Cell Preparations 6. Microscopic Examination of Living Microorganisms Using a Hanging-Drop Preparation or a Wet Mount 7. The Microscopic Measurement of Microorganisms Part 3: Bacterial Staining 8. Preparation of Bacterial Smears 9.
Simple Staining 10. Negative Staining 11. Gram Stain 12. Acid-Fast Stain 13. Differential Staining for Visualization of Bacterial Cell Structures Part 4: Cultivation of Microorganisms: Nutritional and Physical Requirements, and Enumeration of Microbial Poulations 14.
Nutritional Requirements: Media for the Routine Cultivation of Bacteria 15. Use of Differential, Selective, and Enriched Media 16. Physical Factors: Temperature 17. Physical Factors: pH of the Extracellular Environment 18.
Physical Factors: Atmospheric Oxygen Requirements 19. Techniques for the Cultivation of Anaerobic Microorganisms 20.
Serial Dilution - Agar Plate Procedure to Quantitate Viable Cells 21. The Bacterial Growth Curve Part 5: Biochemical Activities of Microorganisms 22. Extracellular Enzymatic Activities of Microorganisms 23. Carbohydrate Fermentation 24. Triple Sugar-Iron Agar Test 25. IMViC Test 26. Hydrogen Sulfide Test 27.
Urease Test 28. Litmus Milk Reactions 29.
Nitrate Reduction Test 30. Catalase Test 31. Oxidase Test 32. Utilization of Amino Acids 33. Genus Identification of Unknown Bacterial Cultures Part 6: The Protozoa 34. Free-Living Protozoa 35.
Parasitic Protozoa Part 7: The Fungi 36. Cultivation and Morphology of Molds 37. Yeast Morphology, Cultural Characteristics, and Reproduction 38. Identification of Unknown Fungi Part 8: The Viruses 39. Cultivation and Enumeration of Bacteriophages 40. Isolation of Coliphages from Raw Sewage Part 9: Physical and Chemical Agents for the Control of Microbial Growth 41.
Physical Agents of Control: Moist Heat 42. Physical Agents of Control: Environmental Osmotic Pressure 43. Physical Agents of Control: Electromagnetic Radiations 44. Chemical Agents of Control: Chemotherapeutic Agents 45.
Determination of Penicillin Activity in the Presence and Absence of Penicillinase 46. Chemical Agents of Control: Disinfectants and Antiseptics Part 10: Microbiology of Food 47. Microbiological Analysis of Food Products: Bacterial Count 48.
Wine Production Part 11: Microbiology of Water 49. Standard Qualitative Analysis of Water 50. Quantitative Analysis of Water: Membrane Filter Method Part 12: Microbiology of Soil 51. Microbial Populations in Soil: Enumeration 52. Isolation of Antibiotic-Producing Microorganisms and Determination of Antimicrobial Spectrum of Isolates 53. Isolation of Pseudomonas Species by Means of the Enrichment Culture Technique Part 13: Bacterial Genetics 53.
Enzyme Induction 54. Bacterial Conjugation 55. Isolation of a Streptomycin-Resistant Mutant 56. The Ames Test: A Bacterial Test System for Chemical Carcinogenicity Part 14: Biotechnology 57.
Bacterial Transformation 58. Isolation of Bacterial Plasmids 59. Restriction Analysis and Electrophoretic Separation of Bacteriophage Lambda DNA Part 15: Medical Microbiology 60. Microbial Flora of the Mouth: Determination of Susceptibility to Dental Caries 61. Normal Microbial Flora of the Throat and Skin 62. Identification of Human Staphylococcal Pathogens 63. Identification of Human Streptococcal Pathogens 64.
Identification of Streptococcus pneumoniae 65. Identification of Enteric Microorganisms Using Computer-Assisted Multitest Microsystems 66. Isolation and Presumptive Identification of Campylobacter 67. Microbiological Analysis of Urine Specimens 68. Microbiological Analysis of Blood Specimens 69.
Species Identification of Unknown Bacterial Cultures Part 16: Immunology 70. Precipitin Reaction: The Ring Test 71.
Agglutination Reaction: The Febrile Antibody 72. Immunofluorescence 73.
Enzyme-Linked Immunoabsorbent Assay 74. Agglutination Reaction: Mono-Test for Infectious Mononucleosis 75. Sexually Transmitted Diseases: Rapid Immunodiagnostic Procedures Appendices.
Key Message: Known for its straightforward and well thought-out laboratory experiments, minimal equipment requirements, and competitive price,Microbiology: A Laboratory Manual, Eighth Editionretains these advantages while gaining currency with a new 'Hot Topics in Microbiology' feature, 50% new color photographs, and a new section of molecular biology experiments. This versatile laboratory manual can be used with any undergraduate microbiology text and course. Key Topics: Basic Laboratory Techniques for Isolation, Cultivation, and Cultural Characterization of Microorganisms; Micros© Bacterial Staining; Cultivation of Microorganisms: Nutritional and Physical Requirements, and Enumeration of Microbial Poulations; Biochemical Activities of Microorganisms; The Protozoa; The Fungi; The Viruses; Physical and Chemical Agents for the Control of Microbial Growth; Microbiology of Food; Microbiology of Water; Microbiology of Soil; Bacterial Genetics; Biotechnology; Medical Microbiology; Immunology Market: For all readers interested in microbiology. Cappuccino is a retired professor (in residence) of Microbiology from the Department of Biology at the State University of New York at Rockland Community College in Suffern, New York. In 1991, he was the recipient of the Chancellor's Award from the State University of New York for Excellence in Teaching. He received his M.S degree (1955) and Ph.D (1957) from Rutgers University, New Brunswick, New Jersey.
From 1957 to 1970 he was associated with the Sloan Kettering Institute for Cancer Research. He was the author and co-author of numerous papers in the area of cancer chemotherapy, and was a member of the faculty of the Sloan Kettering Division of the Graduate School of Medical Sciences at Cornell University in New York, where he taught microbiology. From 1970 to 1995 he taught microbiology, parasitology and clinical chemistry at SUNY Rockland. He is an emeritus member of the American Society for Microbiology (ASM) and an emeritus member of the American Society for Cancer Research (AACR). Although officially retired, he still teaches a microbiology course for nurses at SUNY Rockland. When not writing, he enjoys spending time with his wife Elaine and their family at their summer home at the New Jersey shore.
He also enjoys theater, literature, traveling abroad, and the quiet hours in his wood working shop. Natalie Sherman received her B.S. In biology and her M.S. In microbiology from Fairleigh Dickinson University. Her professional career spanned 32 years at the State University of New York at Rockland Community College in Suffern, New York, as a professor of microbiology.
In addition to microbiology, she taught genetics, anatomy and physiology, and human sexuality. To be more akin to the needs of her students she received an AAS degree in Nursing in 1981. She passed away on October 29, 2001.